Case Study: Stony Brook University maps protein conformations at high throughput with Quantum-Si Next-Gen Protein Sequencing

Stony Brook University maps thousands of protein conformations with Quantum-Si

Stony Brook University faced the challenge of studying non-native, misfolded, or transient protein conformations, which are implicated in diseases like Alzheimer's and cancer but are difficult to detect with traditional methods like crystallography. To overcome the limitations of mass spectrometry for sequencing large variant libraries, they turned to Quantum-Si for its Next-Gen Protein Sequencing (NGPS™) technology on the Platinum® instrument.

Quantum-Si's solution integrated protein barcoding with its single-molecule sequencing technology. This enabled the high-throughput disulfide scanning (HTDS) method to sequence large libraries of short, chemically similar peptide barcodes, a task mass spectrometry could not handle. The result was a dramatic increase in throughput, allowing the screening of thousands of protein conformations compared to fewer than 100 in traditional studies, thereby advancing research in drug discovery and vaccine development.

Stony Brook University