Case Study: Roche achieves rapid, high-throughput LC-UV-MS analysis—cutting analysis time from 5–6 hours to under 30 minutes—with Genedata Expressionist

Automating LC-UV-MS-Based Analytics in Therapeutic Oligonucleotide Process Development

Roche, a leading biopharma developing oligonucleotide therapeutics, faced a major analytical bottleneck in LC‑UV‑MS support for process chemistry: highly complex samples and laborious manual data processing that could take 5–6 hours per sample. To scale throughput and provide timely, comprehensive impurity characterization, Roche partnered with Genedata and deployed Genedata Expressionist for automated MS data processing.

Genedata implemented a workflow-based solution (Genedata Expressionist) that seamlessly imports LC‑UV and MS raw data and automates routine steps such as blank subtraction, spectrum averaging, and MS quantification while preserving expert oversight for unexpected impurities. The automated workflow cut analysis time from 5–6 hours to under 30 minutes per complex sample, increased throughput and impurity identification, and freed MS experts to focus on deeper characterization and method development.

Roche

Tim Nagel

Scientist F.